As a domestic sitcom from the early 1960s, "Pete and Gladys" does not feature any identifiable LGBTQ+ characters or themes. The narrative focuses exclusively on the heterosexual married couple Pete and Gladys Porter and their conventional daily lives, aligning with the social norms and television programming of its era.

The 1960s sitcom 'Pete and Gladys' centers on the everyday lives and comedic predicaments of a suburban married couple. The series does not include any identifiable transsexual characters or explore related themes, consistent with the typical content of mainstream television during that historical period.

The movie does not contain any action or adventure elements.

The main characters, Pete and Gladys Porter, maintain the same genders as established in their prior appearances in "December Bride." No established characters from previous canon or source material had their gender changed for this series.

The show is a spin-off from 'The Danny Thomas Show,' featuring the same lead actors, Harry Morgan and Cara Williams, reprising their roles as Pete and Gladys Porter. Both characters were established as white in the original series and continued to be portrayed by white actors in this show, with no change in racial depiction.