The series features prominent LGBTQ+ characters, particularly Jukebox, whose lesbian identity is depicted with dignity and complexity. Her relationship with Nicole is central to her arc, and while it ends tragically due to external violence, the narrative maintains an empathetic and affirming stance towards queer lives and love, avoiding harmful stereotypes.

Power Book III: Raising Kanan features Jessica, a trans woman, in a significant relationship with Lou-Lou. The show portrays her with dignity, complexity, and agency, challenging stereotypes. While depicting societal prejudice through other characters' transphobic comments, the narrative frames these as external obstacles. Lou-Lou's genuine affection and defense of Jessica contribute to a net positive portrayal, validating trans lives and relationships.