The animated series 'Rambo: The Force of Freedom' does not feature any identifiable LGBTQ+ characters or themes. As a typical 1980s action cartoon, its narrative focuses on military conflicts and heroism, with no elements related to queer identity or experiences. Therefore, the portrayal is categorized as N/A.

The animated series 'Rambo: The Force of Freedom' does not feature any identifiable transsexual characters or themes. Its narrative is centered on action and military conflict, with no elements related to transgender identity.

The animated series features female characters such as Kat, who primarily contribute through espionage, disguise, and technological expertise. There are no clear instances where a female character is depicted defeating one or more male opponents in direct physical combat using martial arts, strength, or melee weapons.

The animated series features John Rambo and Colonel Trautman, who retain their established male genders from the source material. Other significant characters are original to the show and do not have pre-existing canonical genders that were altered.

The animated series features John Rambo, who is consistently portrayed as a white character, aligning with his live-action film counterpart. No established legacy characters from the source material were depicted as a different race.