The show frequently features discussions and monologues that express skepticism and sometimes mockery towards certain LGBTQ+ identities and advocacy efforts, particularly concerning transgender issues. While diverse viewpoints may occasionally be presented, the host's consistent framing often contributes to a problematic portrayal, endorsing ridicule rather than affirmation.

Real Time with Bill Maher frequently discusses transgender themes, often presenting them through a lens of skepticism and criticism. The show's host and guests often question the validity of trans identities and frame related issues as problematic societal trends, leading to a portrayal that is largely dismissive or mocking rather than affirming.

The show, primarily through Bill Maher's commentary, consistently critiques Christianity, portraying its doctrines as irrational and its political influence as detrimental.

Bill Maher's show frequently portrays Islam, particularly its fundamentalist interpretations, as inherently problematic, oppressive, and a source of violence and intolerance.

The show often critiques religious aspects of Judaism, particularly orthodox practices and the intersection of religion with Israeli politics, portraying them as dogmatic or problematic.