Secret Invasion portrays LGBTQ+ themes primarily through subtext and fan interpretation of the close bond between Nick Fury and Talos. While strong chemistry exists, the series does not explicitly depict a queer relationship or explore LGBTQ+ issues directly. This reflects a pattern of limited overt representation, resulting in a neutral impact where identity is present but not central or explicitly affirmed.

The show features G'iah, who engages in and wins close-quarters physical combat against male opponents. This includes a decisive duel where she defeats Gravik using hand-to-hand fighting and powers, and a group fight where she and Varra overcome multiple male foes.

The character Sonya Falsworth, portrayed as female, is an adaptation of the canonically male Marvel Comics character James Falsworth (Union Jack), constituting a gender swap from the source material.

The show subtly incorporates Christian moral themes, such as love overcoming fear and acceptance, through metaphorical narrative elements, aligning the film's message with these virtues.