Shangri-La Frontier, 2023, does not feature any identifiable LGBTQ+ characters or themes. The series focuses entirely on the protagonist's experiences in a virtual reality game, with no narrative elements related to queer identity or relationships.

Based on the available information, there is insufficient data to assess the portrayal of transsexual characters and themes in 'Shangri-La Frontier'. No details regarding such depictions, character arcs, or plot points were provided, making a definitive evaluation impossible at this time.

The anime features powerful female characters like Psyger-0 and Arthur Pencilgon, who are highly skilled combatants in the VRMMORPG. While they are shown defeating numerous monsters and are implied to be top-tier players capable of defeating other players, specific scenes depicting them victorious in close-quarters physical combat against one or more male opponents are not readily identifiable or prominently featured.

The anime adaptation of 'Shangri-La Frontier' faithfully portrays the established genders of its characters from the original web novel and manga. No characters canonically established as one gender in the source material are depicted as a different gender in the on-screen adaptation.

Shangri-La Frontier is a Japanese anime adaptation of a Japanese web novel and manga. The characters, originating from Japanese source material, are portrayed by Japanese voice actors and depicted in a manner consistent with their established background, without any changes to their race.