Southland features Officer John Cooper, a central character whose arc explores his identity as a gay man in law enforcement. The show portrays his coming out, personal struggles, and relationships with dignity and complexity. While addressing external prejudices, the narrative maintains an empathetic stance, affirming the worth of his queer life and experiences.

Southland features Trina, a transgender sex worker, as a victim of a hate crime. The show portrays her with dignity and empathy, framing the violence and prejudice as external forces. The narrative focuses on the police's efforts to seek justice for her, affirming the worth of her life despite tragic circumstances.

Christianity is often portrayed through individual characters finding solace, community, or moral guidance in their faith amidst the harsh realities of police work. The narrative treats these personal beliefs with respect, depicting them as a source of strength or comfort without critiquing the religion itself.