Star Trek: Deep Space Nine does not feature identifiable transsexual characters or themes. While the series explores complex identity through the Trill symbionts inhabiting hosts of different genders, and includes comedic gender-bending plots, these do not directly depict transsexual identity or experiences as defined by the rubric. Therefore, there is no direct portrayal to evaluate.

Star Trek: Deep Space Nine primarily features original characters created for the series. Existing characters carried over from previous Star Trek installments maintain their established genders. No character previously established as one gender is portrayed as a different gender within the show.

Star Trek: Deep Space Nine introduced a new primary cast of characters for the series. These characters were original to DS9 and not re-cast versions of previously established characters from prior Star Trek canon with a different race. Therefore, no race swaps occurred.