The show "StartUp" features a lesbian character, Mara, and her girlfriend, Chloe, whose relationship is depicted respectfully. A key character arc involves Mara's father, Ronald, evolving from initial discomfort to full acceptance and support of his daughter's identity, contributing to an overall affirming portrayal.

The show "StartUp" features Roni, a trans woman, as a central and integral character. She is portrayed with dignity, complexity, and agency, contributing significantly to the plot through her hacking skills. Her trans identity is acknowledged respectfully without being sensationalized or serving as a source of mockery or villainy, resulting in a net positive and affirming portrayal.