The Amazing Digital Circus portrays LGBTQ+ themes positively through characters like Zooble, a non-binary, omnisexual individual whose identity struggles are explored with nuance. The show emphasizes self-acceptance, community, and the 'found family' trope, framing external denial as an obstacle rather than an inherent flaw, leading to an affirming overall impact.

The Amazing Digital Circus portrays gender diversity through Zubel, whose body discomfort and struggle for acceptance metaphorically represent gender dysphoria and non-binary identity. The show's empathetic framing of Zubel's challenges and the use of they/them pronouns contribute to a respectful and affirming depiction, highlighting external obstacles rather than denigrating the character's identity.

The film utilizes Christian symbolism and themes, such as Pomni being likened to Jesus Christ, stigmata, and the Last Supper, to explore narratives of redemption, sacrifice, and a savior figure. These parallels are integrated into the show's core storytelling, aligning with the virtues and narrative power of these Christian concepts.