The show features an affirming portrayal of an LGBTQ+ relationship through Dawn's mother dating a non-binary character, Alex. The depiction is respectful, normalizes the relationship, and integrates Alex positively into the narrative, reflecting the show's overall inclusive tone.

The Baby-Sitters Club features Jamie Newton, a young transgender boy, whose identity is portrayed with dignity and normalcy. The narrative frames his trans identity as an accepted aspect of his character, without it being a source of conflict or drama. This depiction is supportive and validating, contributing to a net positive portrayal.

The 2020 series adapts characters from the original book series. Mary Anne Spier, canonically white in the source material, is portrayed by a Black actress. Dawn Schafer, also canonically white, is portrayed by Latina actresses. These portrayals constitute race swaps.

Jewish characters and traditions, such as Hanukkah and a Bar Mitzvah, are depicted respectfully and integrated naturally into the narrative, highlighting cultural richness and community.