The Golden Girls consistently offered a remarkably progressive and affirming portrayal of LGBTQ+ characters and themes for its era. It depicted gay and lesbian individuals with dignity and complexity, actively challenging prejudice and promoting acceptance through its main characters' empathetic responses and storylines.

The show consistently portrays Christianity as a source of comfort, community, and moral framework for the main characters, particularly through their Catholic and Protestant backgrounds. While individual characters may exhibit human flaws, the narrative itself respects and affirms the role of faith in their lives.

Judaism is depicted respectfully through guest characters and storylines that explore interfaith relationships and cultural differences. The narrative consistently promotes understanding and appreciation for Jewish traditions and beliefs.