The Mindy Project includes several recurring LGBTQ+ characters whose identities and relationships are portrayed positively. The series normalizes same-sex relationships, integrating these characters into the show's comedic world without their identity being a source of ridicule or degradation, thus affirming their dignity and agency.

The Mindy Project features Dr. Jessica Lieberstein, a successful trans woman surgeon portrayed by Laverne Cox. Her character is depicted with dignity and professionalism, serving as a positive representation. The show uses her presence to subtly educate on trans identity through an interaction where she respectfully corrects a character's ignorance, affirming her worth and competence.

Danny Castellano's devout Catholicism is a central aspect of his character, influencing his moral compass, relationships, and life choices. The show portrays his faith with depth and respect, exploring its challenges and virtues without ridiculing the beliefs themselves, even when Mindy's secular views clash with his.

Mindy Lahiri's Indian-American identity is deeply tied to her parents' Hindu cultural and religious background. The show explores the generational and cultural differences between Mindy and her traditional parents, often with humor, but consistently portrays her heritage as a rich and integral part of her identity, without negative judgment.