The animated series 'The New Adventures of Zorro' does not include any identifiable LGBTQ+ characters or themes. The storyline focuses on Zorro's adventures and the fight against injustice in colonial California, without incorporating queer identities or related plot points.

The animated series 'The New Adventures of Zorro' does not include any identifiable transsexual characters or themes. The narrative focuses on traditional adventure elements, and there is no content related to transsexual identities or experiences.

This animated series primarily focuses on Zorro, a male protagonist, engaging in sword fights and physical combat against male antagonists. Female characters in the show are not depicted as participating in or winning direct physical combat against male opponents.

The 1997 animated series maintains the established genders for its core characters, including Zorro/Don Diego, Bernardo, and the main antagonist. No canonical characters from previous Zorro iterations or source material appear to have their gender changed in this adaptation.

The animated series features Zorro (Don Diego de la Vega) and other main characters consistent with their established racial identities as people of Spanish descent in colonial California. No character canonically or historically established as one race is portrayed as a different race.