The Pitt features asexual and seemingly queer female characters within its diverse cast. While explicit LGBTQ+ storylines are not central, the series received top honors at the 2025 Dorian TV Awards from LGBTQ critics, recognizing its broader themes of inclusivity. This suggests a net positive and validating portrayal of the LGBTQ+ community.

The Pitt portrays its transsexual character, Tasha, with respect and dignity. The show avoids common negative tropes, presenting Tasha as a complex and humanized individual acknowledged by medical professionals. This approach contributes to a nuanced and positive representation of trans identity within the hospital drama context.

The show portrays Judaism through a character who, despite personal doubts about faith, finds comfort and solace in Jewish rituals and identity during a panic attack, highlighting its positive role in emotional support and tradition.