The Practice generally offered a positive portrayal of LGBTQ+ characters and themes for its era. It featured a prominent lesbian character, Judge Kittleson, and frequently explored legal battles against discrimination with empathy, framing societal prejudice as an external obstacle rather than an inherent flaw in queer identity. The show consistently advocated for dignity and equality.

The episode 'The Transgendered Client' features a transgender woman as a central character, focusing on her legal defense against murder charges. The narrative frames societal prejudice as the primary obstacle, with the lead attorney advocating for respectful treatment and challenging biases, resulting in a net positive portrayal for its era.

The show frequently explores the moral and ethical dilemmas faced by characters of Christian faith, often portraying their beliefs as a source of strength, comfort, and moral guidance. While it critiques individual hypocrisy or institutional failings, the narrative consistently respects genuine faith and condemns bigotry against it.

Judaism is depicted with respect, often showcasing its cultural and religious significance for characters. The narrative treats Jewish beliefs and practices with dignity, focusing on the individual's experience within the legal and ethical contexts presented.