Titans features a positive portrayal of LGBTQ+ characters, notably through Connor Kent's relationship with Bernard Fitzmartin and Tim Drake's journey of self-discovery. These relationships are depicted with dignity, complexity, and agency, integrated naturally into the narrative. The show affirms the worth of LGBTQ+ lives and love, presenting queer identity as a respected aspect of its characters without relying on harmful stereotypes or making it a source of misery.

Titans includes Vixen (Zoey), a minor transsexual character whose identity is acknowledged without being central to the plot. Her portrayal is incidental, neither strongly affirming nor problematic, presenting her as a normal individual within the narrative.

The show features multiple female characters who are highly proficient in physical combat. Characters like Dawn Granger and Kory Anders are repeatedly shown defeating one or more male opponents through martial arts, physical strength, and melee weapon skills in direct confrontations.

Several key characters, including Starfire, Beast Boy, Rose Wilson, and Jericho, who were canonically depicted as white in the DC Comics source material, are portrayed by actors of different races in the series.