Transformers: EarthSpark features Dot Malto, a main human parent, who is explicitly non-binary and uses they/them pronouns. Their identity is seamlessly integrated into the narrative, presented with dignity and normalcy. Dot is depicted as a capable, loving, and respected individual, contributing to an affirming portrayal of a non-binary character.

Transformers: EarthSpark features Nightshade, a non-binary Transformer whose identity is affirmed through consistent use of they/them pronouns. Nightshade is depicted as a heroic and integral character, contributing positively to the narrative. The show's portrayal is respectful, inclusive, and avoids negative stereotypes, presenting their identity as a natural aspect of their character.