Treme features LaDonna Batiste-Williams, a lesbian character whose identity is portrayed with dignity, complexity, and agency. Her relationships and challenges are depicted realistically, integrated into the broader narrative of post-Katrina New Orleans without sensationalism or negative stereotyping. The show's empathetic stance affirms the worth of her life and love.

Treme features Ladonna, a trans woman whose complex relationship with D.J. Davis and struggles as a sex worker are depicted with empathy. While her story includes violence and a tragic end, the show frames these as external societal issues, maintaining a respectful stance and affirming the worth of her life despite its difficulties.

Treme portrays Christianity, particularly Catholicism, as a vital source of community, resilience, and spiritual solace in post-Katrina New Orleans. The narrative respectfully depicts its role in cultural traditions, funerals, and characters' personal struggles, highlighting its affirming presence.