The series "Twilight of the Gods" integrates LGBTQ+ characters and themes, such as Thyra's bisexuality and explicit homosexual acts, into its mature Norse mythological setting. These identities are presented as integral to the narrative's complex relationships and dramatic fabric, reflecting a sophisticated and unfiltered approach to sexuality without tokenism or negative framing.

Twilight of the Gods offers a highly affirming portrayal of transsexual characters. The Fates are depicted as powerful, central trans/non-binary figures, and Seid-Kona is a strong, integral trans woman. The series integrates these characters respectfully, presenting them with dignity and agency within its mythological framework.

The show features Sigrid, who engages in close-quarters physical combat using melee weapons and hand-to-hand techniques against male gods. Her efforts contribute to their defeat or retreat, and she is explicitly noted as victorious in these engagements.

The animated series features several major Norse mythological characters, such as Loki, Egill, and Hel, who are traditionally depicted as Scandinavian/Northern European. These characters are voiced by actors of African, South Asian, and East Asian descent, respectively, which constitutes a portrayal of a different race than their established origins.

The film portrays Christian symbolism, particularly the crucifixion, as a powerful archetypal image representing universal themes of sacrifice and rebirth. It serves as a narrative device to symbolize the historical transition from Norse paganism to Christianity, treated with thematic depth and respect for its cultural significance rather than theological endorsement or critique.