The series features Liam Walker, a prominent openly gay character whose identity is portrayed positively with dignity and complexity. His relationships are depicted respectfully, and his family's acceptance is a key theme, contributing to an overall affirming portrayal of LGBTQ+ experiences within the narrative.

The show "Walker" features Kai, a recurring trans man character. His identity is handled respectfully, portraying him as a competent, supportive, and well-rounded individual. The narrative integrates his trans identity without making it a source of mockery or misery, thus offering an affirming and dignified representation.

The show features Texas Rangers Micki Ramirez and Cassie Perez, who are frequently depicted engaging in and winning close-quarters physical altercations against male suspects and opponents during investigations and arrests.

The series often portrays Christian values, community, and practices as a positive, grounding force for characters, particularly the Walker family. It aligns with themes of justice, family, and moral integrity without critiquing the faith itself.