X-Men: Evolution does not feature any explicitly identifiable LGBTQ+ characters or themes within its narrative. While the broader X-Men franchise often employs a mutant allegory for marginalized groups, the series itself does not directly explore or depict specific LGBTQ+ identities or issues.

X-Men: Evolution, an animated series centered on teenage mutants, does not include any discernible transsexual characters or themes. The show's narrative primarily explores mutant identity, societal prejudice against mutants, and coming-of-age stories within that context, without touching upon transsexual experiences.

X-Men: Evolution adapts established Marvel Comics characters. All major characters, such as Cyclops, Jean Grey, Wolverine, and Storm, retain their canonical genders from the source material. No instances of a character's gender being changed from their established comic book portrayal are present.

All established X-Men characters in the series retain their canonical race from the source comics. New characters introduced in the show, such as Spyke, do not count as race swaps as they had no prior established race.