The series features prominent LGBTQ+ characters, most notably Mo, a gender-nonconforming, pansexual individual whose journey of self-discovery, relationships, and faith is depicted with depth and affirmation. Other queer characters are present in incidental, non-problematic roles, contributing to a consistently positive and validating portrayal of LGBTQ+ identities.

Zoey's Extraordinary Playlist features Jenna, a trans woman, as a recurring character. Her portrayal is positive, depicting her with dignity, competence, and agency as a software engineer and Max's cousin. The show normalizes her identity respectfully, integrating it into her character without making it a source of conflict or negative stereotypes, thus affirming trans lives.

The series portrays Judaism through Max and his family, highlighting their cultural traditions like Hanukkah with respect and warmth. It integrates Jewish identity as a positive and integral aspect of a main character's background, without critique or negative stereotypes.