Alice in Wonderland (2010) does not feature any identifiable LGBTQ+ characters or themes. The narrative centers on Alice's personal adventure and the fantastical world, with no elements that depict or allude to queer identities or experiences.

Alice in Wonderland (2010) does not feature any identifiable transsexual characters or themes. The film's narrative centers on Alice's return to a fantastical world and her journey of self-discovery, without any plot points or character arcs related to transsexual identity. Consequently, the film has no discernible impact on the portrayal of transsexual individuals.

In the film, Alice Kingsleigh engages in a climactic battle where she defeats the Jabberwocky using a sword. However, this opponent is a fantastical creature, not a male human or humanoid character. No female character is depicted defeating one or more male opponents in close-quarters physical combat.

All major characters from Lewis Carroll's original stories, such as Alice, the Mad Hatter, and the Red Queen, retain their established genders in this adaptation. No significant character's gender was altered from the source material.

The film adapts Lewis Carroll's classic stories, where main characters like Alice, the Mad Hatter, and the Queens are traditionally depicted as white. The 2010 adaptation portrays these characters with white actors, aligning with their established racial depictions.