This family-oriented animated film does not include any discernible LGBTQ+ characters or themes. The narrative focuses on the adventures of Alvin, Simon, and Theodore, with no elements related to queer identity present in the story.

The film "Alvin and the Chipmunks: The Road Chip" does not feature any identifiable transsexual characters or themes within its narrative. The plot focuses on the adventures of the chipmunks and their human companions, without engaging with transgender identity or related issues.

The film does not feature any scenes where a female character, such as Brittany, Jeanette, Eleanor, or any other, engages in and wins close-quarters physical combat against one or more male opponents. The female characters are not portrayed in roles involving direct physical confrontation or martial arts.

The film features established characters like Alvin, Simon, Theodore, Dave, and The Chipettes, whose genders remain consistent with their long-standing canonical portrayals. No existing characters were depicted with a different gender.

The main characters are anthropomorphic chipmunks, to whom the concept of human race does not apply. Human characters like Dave Seville maintain their established racial portrayals from previous installments. No legacy character's race was altered.