The film 'Ayyappanum Koshiyum' does not include any identifiable LGBTQ+ characters or themes in its narrative. The story focuses on other aspects without engaging with queer identity or related storylines.

Ayyappanum Koshiyum does not feature identifiable transsexual characters or themes. The story primarily focuses on the escalating conflict between two men, Ayyappan Nair and Koshy Kurien, and their personal battles within a rural setting. The narrative does not include any elements related to transsexual identity or experiences.

The film centers on the intense conflict between its male protagonists. While female characters are present in significant supporting roles, none are depicted engaging in or achieving victory in direct physical combat against male opponents. The narrative does not feature any female characters winning hand-to-hand confrontations or martial arts contests.

Ayyappanum Koshiyum is an original film without prior source material or established characters. Consequently, no characters exist who were canonically or historically established as a different gender before this production. The film does not feature any gender swaps.

The film features original characters created specifically for this production. There is no prior source material or historical record establishing their race before this film's release, meaning no race swap occurred.