The film's central conflict involves the Barbies reclaiming Barbie Land from the Kens. This is achieved through strategic social manipulation and intellectual maneuvering, leading the Kens to fight among themselves. There are no scenes depicting female characters engaging in or winning direct physical combat against male opponents.

The film adapts the established Barbie and Ken doll characters, along with other associated figures from the toy line and real-world history, maintaining their original genders as depicted in the source material. No character established as one gender is portrayed as a different gender.

The film features multiple Barbies and Kens of diverse races, reflecting the varied doll line. However, no specific, named character with a previously established race (from source material or prior adaptations) is portrayed by an actor of a different race.