Bottoms offers a largely positive portrayal of LGBTQ+ characters, particularly lesbians, by normalizing their experiences within a high school comedy. It depicts multi-dimensional, flawed protagonists who experience queer joy and empowerment, diverging from tragic narratives. The satirical approach critiques societal norms while affirming queer identity and relationships.

"Bottoms" includes a brief, positive depiction of trans women who are portrayed as strong and empowered in a scene where they confront bigoted individuals. While this contributes to queer visibility and empowerment, the film does not delve deeply into trans issues or offer a comprehensive exploration of trans characters, focusing instead on its lesbian protagonists. The net impact is positive due to the affirming nature of the depiction, despite its limited scope.