The film contains no depiction of LGBTQ+ characters or themes, focusing instead on themes of collective resilience and crisis management among a predominantly straight, Japanese ensemble.

No transgender characters or themes appear in the film. The story unfolds as an action thriller involving a high-stakes bomb on a speeding train, with character arcs focused on survival and crisis management among a diverse but non-transgender cast.

Female characters such as the train driver Chika Matsumoto, politician Yuko Kagami, student Yuzuki Onodera, and teacher Sakura Ichikawa contribute to resolving the bomb crisis through driving, calming passengers, and revelations. No scenes depict them victorious in close-quarters physical combat against male opponents.

Bullet Train Explosion presents original characters in a contemporary Japanese disaster thriller context, with all principal roles cast with Japanese actors consistent with the characters' established ethnicities. No source material, prior adaptations, or historical figures introduce any racial discrepancies.