The film 'Lone Wolf and Cub: Sword of Vengeance' does not feature any identifiable LGBTQ+ characters or themes. Its narrative is centered on the samurai Itto Ogami's quest for revenge and his journey with his son, without incorporating any elements related to queer identity.

Based on available information, 'Lone Wolf and Cub: Sword of Vengeance' does not feature any identifiable transsexual characters or themes. The film's plot centers on a samurai's quest for revenge, and there are no narrative elements that depict or engage with transsexual identities.

The film primarily focuses on the male protagonist, Ogami Itto, and his battles against male samurai. While female characters are present in the narrative, none are depicted engaging in or winning direct physical combat against one or more male opponents.

The 1972 film "Lone Wolf and Cub: Sword of Vengeance" adapts the original manga series. All major characters, including Ogami Ittō and Daigorō, maintain their canonical male gender from the source material in this adaptation.

The 1972 film "Lone Wolf and Cub: Sword of Vengeance" is a Japanese adaptation of a Japanese manga, featuring Japanese actors portraying canonically Japanese characters. There is no evidence of any character's race being changed from the source material.