But I'm a Cheerleader offers a highly affirming portrayal of LGBTQ+ characters and themes. It satirizes conversion therapy while celebrating self-discovery, queer love, and community. The narrative champions authentic identity against societal pressures, concluding with a positive embrace of LGBTQ+ lives.

But I'm a Cheerleader features Dolph, a trans man, whose identity is depicted with dignity and agency. The film critiques conversion therapy's attempts to alter his gender identity, framing these efforts as misguided. Dolph's arc is affirming, as he confidently asserts himself and leaves the camp.

The film satirizes a specific, conservative interpretation of Christianity, portraying its institutions and adherents as hypocritical, oppressive, and foolish in their attempts to 'cure' homosexuality. The narrative highlights the harm caused by this ideology without offering counterbalancing positive portrayals.