Despicable Me 3 does not include any identifiable LGBTQ+ characters or themes. The narrative focuses on Gru, his family, and his twin brother, with no elements related to queer identity present in the film's plot or character development.

Despicable Me 3 does not feature any identifiable transsexual characters or themes. The narrative focuses on Gru's family dynamics, his twin brother Dru, and their conflict with the villain Balthazar Bratt, without engaging with transgender identity or related issues.

The film features Lucy Wilde, an AVL agent, who participates in action sequences using gadgets and agility. However, there are no clear instances where she defeats one or more male opponents in direct, close-quarters physical combat using skill, strength, or martial arts.

Despicable Me 3 features returning characters who maintain their established genders from previous installments. New characters introduced in this film do not have prior canonical genders to be swapped. Therefore, no gender swaps occur.

Despicable Me 3 is a direct sequel in an animated franchise. All established characters maintain their original visual depictions and implied racial characteristics from previous installments. No character's race was altered from prior canon.