Detective Conan: The Scarlet Bullet does not feature any identifiable LGBTQ+ characters or themes. The film's narrative is entirely focused on its mystery plot, action sequences, and the established heterosexual relationships of its main cast, resulting in no LGBTQ+ portrayal.

The film "Detective Conan: The Scarlet Bullet" does not feature any identifiable transsexual characters or themes. Its narrative focuses on a mystery involving a major sporting event and a new bullet train, without engaging with issues related to trans identity or experience.

While female characters like Ran Mouri and Masumi Sera possess combat skills, the film does not feature any scenes where they directly defeat one or more male opponents in close-quarters physical combat. Their action sequences are primarily defensive or involve overcoming environmental obstacles.

The film features established characters from the long-running Detective Conan franchise, all of whom maintain their canonical genders from the source manga and anime. No characters historically or widely established as one gender are portrayed as a different gender.

All established characters in "Detective Conan: The Scarlet Bullet" are portrayed consistent with their canonical race and ethnicity from the source manga and anime. No existing character was depicted as a different race.