Dirty Grandpa features LGBTQ+ elements predominantly as sources of crude humor and shock. The film employs harmful stereotypes, depicting characters and situations involving queer identity or gender non-conformity for mockery and degradation, with no counterbalancing positive portrayal.

Dirty Grandpa" features a comedic plot point where a character is led to believe they had a sexual encounter with a trans woman, causing panic. The film derives humor from this premise, playing on the character's discomfort and reinforcing negative stereotypes about trans identity as a source of shock or ridicule, without offering any counterbalancing positive portrayal or critique.

The film portrays Christianity through the character of Meredith and her family, depicting them as hypocritical, judgmental, and controlling. Their outward piety is contrasted with their materialistic and manipulative behavior, serving as a target for satire.