The Guardians of the Galaxy Holiday Special does not feature any explicit LGBTQ+ characters or themes. The narrative focuses on the Guardians' efforts to bring joy to Peter Quill, without incorporating any queer identities or relationships.

The film does not include any identifiable transsexual characters or themes. Consequently, there is no portrayal to evaluate, resulting in a 'N/A' rating for its net impact on transsexual representation.

The special features female characters like Mantis and Nebula. Mantis primarily uses her empathic powers to incapacitate or calm individuals, which falls outside the criteria for physical combat. Nebula does not engage in direct physical combat against male opponents.

All major and legacy characters in The Guardians of the Galaxy Holiday Special are portrayed consistently with their established racial or species-based depictions from prior source material or previous installments. No character originally established as one race is portrayed as a different race.