Guardians of the Galaxy does not include any identifiable LGBTQ+ characters or themes. The narrative focuses on its main ensemble's individual backstories and their formation as a team, without incorporating elements of queer identity or relationships.

Guardians of the Galaxy does not feature any identifiable transsexual characters or themes within its narrative. Consequently, there is no portrayal to evaluate, resulting in an N/A rating for its depiction of transsexual individuals or themes.

All major and supporting characters adapted from Marvel Comics for Guardians of the Galaxy (2014) retain their established canonical gender from the source material. No instances of a character being portrayed as a different gender were identified.

The film primarily features alien characters whose species defines their appearance, not human racial categories. The main human character, Peter Quill, maintains his established race from the source material. No instances of a character canonically established as one human race being portrayed as a different race were identified.