The film includes extremely minor, background LGBTQ+ representation through a brief visual of an implied same-sex alien couple. This depiction is incidental, not central to the plot or character development, and neither affirms nor denigrates queer identity, resulting in a neutral overall impact.

The film features female characters such as Nebula and Gamora who engage in and win close-quarters physical combat against multiple male opponents, utilizing their strength, skills, and melee weapons.

The character of the High Evolutionary, canonically depicted as Herbert Wyndham, a white man in Marvel Comics, is portrayed by Chukwudi Iwuji, a Black actor, in the film. This constitutes a race swap.