Due to a lack of available information regarding the plot, characters, and themes of 'Dooba Dooba, 2024', an accurate assessment of its portrayal of LGBTQ+ characters and themes cannot be made. There is insufficient data to determine if any LGBTQ+ elements are present or their nature.

Based on the provided information, there is not enough detail about the film's narrative or characters to determine if transsexual individuals or themes are present, or to evaluate their portrayal. Therefore, a comprehensive assessment of the net impact is not possible at this time.

The movie does not contain any action or adventure elements.

Without information on source material or character origins, it is impossible to determine if any characters in 'Dooba Dooba, 2024' were canonically established with a different gender prior to this film.

The film "Dooba Dooba, 2024" is presented without any prior source material, historical context, or previous adaptations. Therefore, there is no established canonical or historical race for its characters to compare against the on-screen portrayals.