The film 'Escape Room: Tournament of Champions' does not feature any identifiable LGBTQ+ characters or themes. The narrative focuses solely on the survival of its contestants through a series of deadly escape rooms, with no elements related to queer identity present in the plot or character arcs.

The film 'Escape Room: Tournament of Champions' does not feature any identifiable transsexual characters or themes within its narrative. Consequently, there is no portrayal to assess regarding positive, negative, or neutral impact.

The film focuses on characters solving elaborate puzzles and surviving environmental traps. There are no instances where a female character engages in and wins direct physical combat against one or more male opponents using skill, strength, or martial arts.

This film is a direct sequel to an original movie series. All returning characters maintain their established gender, and new characters introduced are not gender-swapped versions of previously established figures from any prior canon.

This film is a sequel to an original story, not an adaptation of pre-existing material. All returning characters maintain their established race from the previous installment, and new characters are original to this film, thus having no prior canonical race to be swapped from.