Ghostbusters: Afterlife does not feature any identifiable LGBTQ+ characters or themes, nor does it explore any queer-related narratives. Therefore, its net impact on LGBTQ+ portrayal is not applicable.

Ghostbusters: Afterlife does not feature any identifiable transsexual characters or themes. The story centers on the Spengler family's legacy and their battle against supernatural forces, with no elements related to transgender identity present in the plot or character development.

The film primarily features female characters, such as Phoebe Spengler, engaging in combat against supernatural entities (ghosts) using specialized ghost-busting equipment. There are no instances where a female character defeats one or more male opponents in direct physical combat, such as hand-to-hand or melee weapon fights.

The film introduces new, original characters who are descendants of the original Ghostbusters. Legacy characters who appear retain their established genders from previous installments. No established character undergoes a gender change.

Ghostbusters: Afterlife introduces new characters who are descendants of Egon Spengler, but these are original to the film. All returning legacy characters maintain their established races from previous installments. No character canonically established as one race was portrayed as a different race.