Gold Rush Gang does not explicitly feature or prominently portray LGBTQ+ characters or themes. The plot centers on wartime adventure, romance, and camaraderie among its primarily heterosexual characters, with no indication of LGBTQ+ identities or issues being addressed within its storyline.

The film 'Gold Rush Gang' does not feature transsexual characters or themes in its narrative, according to available information, official synopses, and critical reviews. The plot focuses on a gold train heist during WWII, emphasizing action, comedy, and rivalry. Consequently, the film receives an N/A rating for its portrayal of transsexual individuals or communities.

The film's main characters, based on Thai folklore and history, are portrayed consistent with their established canonical genders. There is no indication of any character having an on-screen gender differing from their source material or historical record.

The film's casting predominantly features Thai actors, and the inclusion of mixed-ethnicity actors is presented as historically plausible for the WWII Thailand setting. No character established as one race is portrayed as a different race.