The film does not feature any identifiable LGBTQ+ characters or themes. The narrative focuses on the coming-of-age story of three male friends and their summer adventure, without exploring queer identities or relationships within its plot or character arcs.

The film "Goodbye, Don Glees!" does not feature any identifiable transsexual characters or themes. The narrative centers on a coming-of-age story about three cisgender male friends and their adventures, without engaging with topics of gender identity or transition.

The film focuses on the coming-of-age story of three male friends and their summer adventures. There are no significant female characters depicted in any action or combat roles, nor are there any scenes where a female character engages in or wins a physical fight against male opponents.

The film is an original animated production, not an adaptation or reboot of pre-existing material. All characters introduced are new creations for this specific story, meaning there are no prior established character genders to compare against for a swap.

The film is an original animated story with no prior source material, historical figures, or legacy characters. All characters were created for this specific film, meaning there is no established baseline race from which to deviate.

Not depicted in the film.