Isle of Dogs does not include any identifiable LGBTQ+ characters or themes. The narrative focuses on a boy's quest to find his dog amidst a canine exile, exploring themes of loyalty, political corruption, and human-animal bonds without engaging with queer identity.

Isle of Dogs does not feature any identifiable transsexual characters or themes. The narrative is centered on a dystopian future where dogs are exiled, and a boy's quest to find his pet, with no elements pertaining to transgender identity present in the story or character portrayals.

The film features several female characters, both human and canine. While the dogs participate in group confrontations, no individual female character is depicted as physically defeating one or more male opponents in close-quarters combat. Human female characters are not involved in physical combat.

Isle of Dogs is an original story with no pre-existing source material, prior installments, or historical figures. All characters were created for this film, meaning none had a previously established gender to be swapped.

Isle of Dogs is an original story with characters created specifically for the film. There is no prior source material or established canon where any character's race was different from their portrayal in the movie.