The film features multiple female characters, including Hit-Girl and Mother Russia, who engage in and win close-quarters physical fights against numerous male opponents, demonstrating superior combat skills and strength.

The character Doctor Gravity, depicted as white in the original comic book source material, is portrayed by a Black actor in the film adaptation.

The film portrays Colonel Stars and Stripes as a born-again Christian whose faith genuinely motivates him to do good and adopt a moral code, transforming him from a violent past. While the narrative highlights the inherent contradictions of a 'Christian vigilante' through satire, it does not ridicule Christianity itself, but rather the character's struggle to reconcile his beliefs with his violent actions. His faith is presented as a positive personal influence.