One Piece: Dead End Adventure does not feature any identifiable LGBTQ+ characters or themes. Consequently, there is no portrayal of queer identity or related narratives within the film's plot or character arcs, leading to a determination of N/A for its net impact.

The film "One Piece: Dead End Adventure" does not feature any identifiable transsexual characters or themes. Its plot centers on an adventure involving the Straw Hat Pirates participating in a dangerous race, with no narrative elements touching upon transgender identity or experiences.

The film features female characters Nami and Nico Robin who engage in combat. Nami primarily uses her Clima-Tact for weather manipulation, and Robin utilizes her Devil Fruit power to sprout body parts. Their victories are achieved through these unique abilities, which do not align with direct physical combat based on skill, strength, or martial arts as defined.

The film features the established Straw Hat Pirates crew, all of whom retain their canonical genders from the One Piece manga and anime. New characters introduced in the movie are original and not gender-swapped versions of pre-existing characters.

This 2003 animated film is a direct continuation of the established One Piece anime series. It maintains the visual designs and racial portrayals of its existing characters from the source manga and prior animated installments, without altering any established racial characteristics.