No LGBTQ+ characters or themes are present in the film. The story examines the evangelical journey of John Allen Chau without addressing queer identity or representation.

The film contains no depiction of transsexual characters or themes. Its narrative explores the final days of a Christian missionary without addressing transgender identity or related elements.

Female characters appear in supporting missionary roles, but the biographical drama contains no scenes of them defeating male opponents in close-quarters physical combat. The narrative focuses on a male protagonist's journey and tragic end without action sequences matching the criteria.

Last Days depicts the true story of male missionary John Allen Chau and his family, with all historical figures portrayed by actors of the same gender. Supporting roles are either fictional or match real-life genders, resulting in no gender swaps.

The biopic depicts John Allen Chau, historically of mixed Chinese and white ancestry, played by Sky Yang of similar mixed heritage. His Chinese father is portrayed by Chinese-American actor Ken Leung, and white mother by white actress Claire Price. Fictional supporting roles lack prior racial baselines, resulting in no race swaps.