Based on available information, Madame Web (2024) does not feature explicit LGBTQ+ characters or themes. The film's focus is primarily on female empowerment and diversity in terms of race and gender, with no reported LGBTQ+ representation or storylines. Therefore, the net impact on LGBTQ+ portrayal is N/A.

Madame Web (2024) does not feature any identifiable transsexual characters or themes within its narrative. Based on available information, there is no depiction or direct portrayal of the transsexual community in the film's plot, characterizations, or themes.

The film features female characters, including Cassie Webb and the three young women she protects, who engage in combat against male opponents. However, their victories are consistently achieved through the use of their developing superpowers, such as precognition, super strength, enhanced agility, and camouflage, rather than non-superpowered physical skill or martial arts.

The film features female leads who are canonically female in the source material, such as Cassandra Webb (Madame Web), Julia Carpenter, Anya Corazon, and Mattie Franklin (all Spider-Women). The male antagonist, Ezekiel Sims, also retains his canonical gender. No established character's gender was changed for this adaptation.