Due to a lack of specific information regarding the film's content, it is not possible to determine if LGBTQ+ characters or themes are present, or to evaluate their portrayal. Therefore, an assessment of the net impact cannot be made.

There is not enough information available to assess the film's portrayal of transsexual characters or themes. Without plot details or character descriptions, it is impossible to determine if such depictions exist or what their nature might be, thus preventing any evaluation of net impact.

The film is an animated slapstick comedy. While female characters like Ofelia engage in comedic, exaggerated physical interactions, these do not depict instances of skilled physical combat where a female character defeats one or more male opponents through martial arts or strategic strength.

The film adapts the long-running Spanish comic series 'Mortadelo y Filemón.' All major characters from the source material, such as Mortadelo, Filemón, and Ofelia, retain their established genders in the 2014 animated adaptation.

Mortadelo and Filemón, along with other characters from the original Spanish comic series, are consistently depicted as white. The 2014 film adaptation features a cast of white actors portraying these characters, aligning with their established racial depictions.