The film does not feature any explicitly identifiable LGBTQ+ characters or themes. The narrative focuses on friendship, music, and magical conflicts, with no elements suggesting queer identities or relationships, resulting in no LGBTQ+ portrayal.

The film 'My Little Pony - Equestria Girls - Rainbow Rocks' does not feature any identifiable transsexual characters or themes. Its plot centers on high school life, musical battles, and magical elements, with no narrative elements touching upon gender identity.

The movie does not contain any action or adventure elements.

The film adapts established female pony characters from the My Little Pony franchise into human girls. Their gender identity remains consistently female across both portrayals, with no character established as one gender being depicted as a different gender.

The film features characters who are human-like counterparts of ponies from the My Little Pony franchise. As the original characters are ponies, they do not have a human race to be swapped from. The human-like designs are consistent with their established appearances within the Equestria Girls series.