Based on available information, Night (2024) does not include any explicit LGBTQ+ characters, storylines, or themes. The film's narrative centers on a mother's search for her child in a war-torn town, focusing on universal themes of loss, trauma, and resilience, rather than issues of sexual orientation or gender identity.

Due to the unavailability of specific plot details or character information for the film 'Night' (tt14174036, 846817), an evaluation of its portrayal of transsexual characters and themes cannot be performed. Therefore, the net impact is determined as N/A, indicating no identifiable depiction.

The movie does not contain any action or adventure elements.

Night is an original animated film without prior source material or historical characters. Consequently, no character's on-screen gender deviates from a previously established canonical or historical gender.

The film's characters and setting are established as Palestinian, and the voice actors align ethnically and phenotypically with this context. There is no indication that any character, canonically or historically, was of a different race than portrayed.